Figure 6.

Schematics of a multiwell, dual chamber bioreactor system (modified from Lozito et al.²³³). On the left, a representation of a single bioreactor well. A removable, non-permeable insert (b) hosts the chondral tissue (d) and the bone tissue (e) constructs. The insert with the osteochondral construct is placed within a well of the bioreactor platform as shown on the right, and the O-rings (c) seal the separation of the well in an upper and lower chamber. A stream of chondrogenic medium (yellow) is perfused through the upper compartments in communication with the chondral tissue, while osteogenic medium (orange) is perfused through the lower chamber in communication with the bone tissue. Sealing is further ensured by a lid (a) equipped with an O-ring (c). On the right, a representation of the multiwell bioreactor platform for the simultaneous culture of 96 osteochondral constructs. The multiwell platform replicates the well dimensions and arrangement of standard 96-well tissue culture plates. Representative inserts and sealing lids with O-rings depicted in red are shown. The microfluidics connects eight wells in each column allowing for averaging of the experimental conditions along each array. (A color version of this figure is available in the online journal.)