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. 2017 Apr 21;8:15055. doi: 10.1038/ncomms15055

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Overview of small molecules and growth factors supplemented in NCM, NHSM and RT naive media. (b) Morphological analysis of hESC cultures in different naive media and their transition from primed pluripotency-specific flat morphology towards dome-shaped undifferentiated naive colonies. NCM and NHSM media comprised of several domed colonies whereas their frequency was lower in RT media. Scale bars, 200 μm.