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. 2017 Apr 21;8:15055. doi: 10.1038/ncomms15055

Figure 2. Assessment of naive pluripotency in the newly formed naive hESCs from primed hESC line UGent11-70.

Figure 2

(a) Doubling time and single-cell clonogenicity assays for primed hESCs, NCM-, NHSM- and RT-naive hESCs. a* compared to a and b* compared to b, *P<0.05. (b) Depiction of chromosomal analysis for primed hESCs, NCM-, NHSM- and RT-naive hESCs via array comparative genome hybridization (aCGH). (c) Representative immunofluorescence image for pluripotency makers OCT4 and NANOG in NCM-, NHSM- and RT-naive hESCs; Scale bars, 200 μm. (d) qRT-PCR analysis for ectoderm, mesoderm and endoderm markers on spontaneously differentiated primed hESCs, NCM-, NHSM- and RT-naive hESCs as EBs for 14 days. (e) Gene expression analysis for naive-pluripotency specific genes on undifferentiated primed hESCs, NCM-, NHSM- and RT-naive hESCs. a* compared to a and b* compared to b, *P<0.05. The data are represented as mean±s.d.