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. 2017 Apr 21;8:15055. doi: 10.1038/ncomms15055

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Gene expression analysis for lineage-specific genes upon directed differentiation of naive hESC line UGent11-70 towards (a) ectoderm, (b) mesoderm and (c) endoderm. All germ layer markers were analysed irrespective of the targeted lineage to determine the heterogeneity in differentiation between the different naive medium conditions as well as between primed and naive conditions. Dashed box represents the gene expression for markers specific for that lineage. The data are represented as mean±s.d.