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Genetic complementation of strain PKGI,II-mAID-3HA. (A) Schematic of the CRISPR/Cas9 strategy used to insert a second copy of PKG or mutant isoforms of pkg into the UPRT locus of PKGI,II-mAID-3HA parasites. dhfr-ts*, Pyrr allele. (B) Coexpression of PKGI,II-mAID-3HA (both green) and PKGI,II-6Ty (red) constructs assessed by IF microscopy. Scale bars = 5 µm.
