Table 1.
Description of post-mortem tissue samples collected at RVLs
| Calf ID | Tissue types collected | Preservation method | Age (months) | Sex | Breed | County of farm of origin | RVL location | Lesions observed | Histology | RVL diagnosis |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | lung | frozen | 6 | F | SIX | Donegal | Sligo | ✓ | ✓ | Pneumonia (BRSV suspected) |
| 2 | lung | frozen | 1 | M | FRX | Donegal | Sligo | ✓ | x | Pneumonia and enteritis |
| 3 | lung | frozen | 12 | M | PTX | Donegal | Sligo | ✓ | ✓ | Pneumonia - IBR and M. bovis |
| 4 | lung | frozen | 12 | M | BBX | Donegal | Sligo | ✓ | ✓ | Pneumonia - IBR and M. bovis |
| 5 | lung | frozen | 7 | M | CHX | Donegal | Sligo | ✓ | ✓ | Pneumonia - H. somni |
| 6 | lung | frozen | 6 | F | CHX | Leitrim | Sligo | ✓ | x | Pneumonia - M. haemolytica and BPIV-3 virus |
| 7 | lung and lymph node | frozen | 1 | F | LM | Sligo | Sligo | ✓ | x | Pneumonia and navel infection - M. bovis, Pseudomonas spp. |
| 8 | lung and lymph node | RNALater | 8 | M | FR | Leitrim | Sligo | ✓ | x | Pneumonia - T. pyrogenes |
| 9 | lung and lymph node | RNALater | 0 | M | RBX | Donegal | Sligo | ✓ | x | Pneumonia - IBR, H. somni,
T. pyrogenes |
| 10 | lung and lymph node | RNALater | 3.5 | M | FR | Leitrim | Sligo | ✓ | x | Pneumonia- P. multocida andS. dublin |
| 11 | lung and lymph node | RNALater | 2.5 | F | LM | Sligo | Sligo | ✓ | x | Pneumonia |
| 12 | lung and lymph node | RNALater | 0 | F | CHX | Carlow | Kilkenny | ✓ | x | Pneumonia |
| 13 | lung and lymph node | RNALater | 3 | F | AAX | Galway | Athlone | ✓ | ✓ | Pneumonia - M. haemolytica |
| 14 | lung and lymph node | RNALater | 2 | F | LMX | Westmeath | Athlone | ✓ | ✓ | Pneumonia and bacteraemia-P. multocida |
| 15 | lung and lymph node | RNALater | 0 | F | AAX | Offaly | Athlone | ✓ | ✓ | Pneumonia - IBR and necroticlaryngitis |
| 16 | lung and lymph node | RNALater | 0 | F | AAX | Offaly | Athlone | ✓ | ✓ | Pneumonia and pleurisy -Pasteurella spp., IBR and BRSV |
| 17 | lung and lymph node | RNALater | 0 | M | CHX | Longford | Athlone | ✓ | ✓ | Pneumonia and rotavirusenteritis |
| 18 | lung and lymph node | RNALater | 1 | M | CHX | Roscommon | Athlone | ✓ | ✓ | Pneumonia and navel ill -T. pyogenes (suspected virus and Pasteurella) |
| 19 | lung and lymph node | RNALater | 0 | M | WAX | Waterford | Kilkenny | ✓ | x | Pneumonia and rotavirus enteritis |
| 20 | lung and lymph node | RNALater | 1.5 | F | FR | Tipperary | Kilkenny | ✓ | ✓ | Pneumonia - H. somni andM. bovis |
| 21 | lung and lymph node | RNALater | 3 | M | LM | Kilkenny | Kilkenny | ✓ | ✓ | Pneumonia - H. somni andM. bovis |
| 22 | lung and lymph node | RNALater | 0 | F | CHX | Kilkenny | Kilkenny | ✓ | ✓ | Pneumonia - P. multocida /H. somni bronchopneumonia |
| 23 | lung and lymph node | RNALater | 0 | M | LMX | Laois | Kilkenny | ✓ | ✓ | Pneumonia - H. somni bronchopneumonia |
| 24 | lung and lymph node | RNALater | 3 | M | LMX | Sligo | Sligo | ✓ | x | Pneumonia |
| 25 | lung and lymph node | RNALater | 2 | M | CH | Mayo | Sligo | ✓ | x | Pneumonia - Proteus spp. |
| 26 | lung and lymph node | RNALater | 3 | F | CHX | Leitrim | Sligo | ✓ | x | Pneumonia - Endocarditis, enzootic pneumonia, inhalation pneumonia |
| 27 | lung and lymph node | RNALater | 1.5 | F | LMX | Sligo | Sligo | ✓ | x | Pneumonia - IBR |
| 28 | lung and lymph node | RNALater | 1.5 | F | AAX | Sligo | Sligo | ✓ | x | Pneumonia - IBR and Proteus spp. |
| 29 | lung and lymph node | RNALater | 12 | M | CHX | Donegal | Sligo | ✓ | ✓ | Pneumonia - (possible IBR) and meningitis |
| 30 | lung and lymph node | RNALater | 1 | M | AA | Sligo | Sligo | ✓ | x | Pneumonia - M. bovis |
| 31 | lung and lymph node | RNALater | 2.5 | F | LMX | Donegal | Sligo | ✓ | x | Pneumonia - M. bovis |
| 32 | lung and lymph node | RNALater | 3 | F | LMX | Kilkenny | Kilkenny | ✓ | ✓ | Pneumonia - interstitial pneumonia |
| 33 | lung and lymph node | RNALater | 2.5 | M | FR | Laois | Kilkenny | ✓ | x | Pneumonia - P. multocida |
| 34 | lung and lymph node | RNALater | 5 | M | AAX | Kilkenny | Kilkenny | ✓ | ✓ | Pneumonia - P. multocida, T. pyrogenes chronic bronchopneumonia |
| 35 | lung and lymph node | RNALater | 4.5 | F | LMX | Sligo | Sligo | ✓ | ✓ | Pneumonia - Pasteurellosis |
| 36 | lung and lymph node | RNALater | 1 | M | CHX | Longford | Athlone | ✓ | ✓ | Pneumonia - suppurative bronchial pneumonia. |
| 37 | lung and lymph node | RNALater | 1.5 | M | CHX | Galway | Athlone | ✓ | ✓ | Pneumonia - M. haemolytica |
| 38 | lung and lymph node | RNALater | 3.5 | F | AAX | Offaly | Athlone | ✓ | ✓ | Pneumonia - H. somni, M. bovis, M. haemolytica, P. multocida, T. pyogenes |
Lesions were defined as macroscopic consolidation or abscessation of lung tissue.
M male, F female, AA Aberdeen Angus, AAX Aberdeen Angus cross, BBX Belgium Blue cross, CH Charolais, CHX Charolais cross, FR Friesian, FRX Friesian cross, LM Limousin, LMX Limousin cross, PTX Parthenaise cross, RBX Rotbunt cross, SIX simmental cross, WAX wagyu cross, ✓ = performed and confirmed bovine respiratory disease, x = not performed, H. somni = Histophilus somni, M. bovis = Mycoplasma bovis, M. haemolytica = Mannheimia haemolytica, P. multocida = Pasteurella multocida, S. dublin = Salmonella dublin, T. pyrogenes = Trueperella pyrogenes, BRSV = bovine respiratory syncytial virus, IBR = infectious bovine rhinotracheitis, BPIV-3 = bovine parainfluenza 3 virus.