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. 2017 Apr 27;8:15164. doi: 10.1038/ncomms15164

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Copyright © 2017, The Author(s)

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(a) Cdc6 colocalizes with Plk4. U2OS cells were co-transfected with mCherry-tagged Cdc6 (upper panel) or Myc-tagged Cdc6 (lower panel) with GFP-tagged Plk4. Myc-tagged Cdc6 was stained with a Myc antibody. (b) Cdc6 colocalizes with Plk4 and Sas-6 viewed by 3D-SIM. 3D-SIM images of U2OS cells transfected with GFP-tagged Plk4 (upper panel) or GFP-tagged Sas-6 (lower panel) and stained for endogenous Cdc6 and CP110. (c–f) Cdc6 colocalizes with Plk4, STIL and Sas-6 viewed by STED. Immunofluorescence of endogenous Cdc6 (green) and Plk4 (red), or Cdc6 (green) and STIL (red) in U2OS cells (c,e). Immunofluorescence of endogenous Cdc6 (red) in U2OS cells transfected with GFP-tagged Plk4 or GFP-tagged Sas-6 (d,f). (g) Schematic diagrams illustrating the localization of proteins at the centrosome in b–f. Different proteins are indicated by the colours of staining in b–f. DNA was stained with DAPI. Scale bars in a,c–f, 10 μm. Scale bar in b, 0.2 μm. Insets in a,c–f are high-magnification views of the regions indicated in the low-magnification images.