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. 2017 Apr 27;8:15034. doi: 10.1038/ncomms15034

Figure 3. Regulatory activity for the genomic region containing rs36115365.

Figure 3

(a) Allele-specific luciferase activity was tested for rs36115365 in four cancer derived cell lines from pancreas (PANC-1) (b) melanoma (UACC903) (c) testis (NTERA-2) and (d) lung (A549). DNA fragments containing either the C or G allele at rs36115365 were cloned upstream of a minimal promoter (pGL4.23) driven reporter in the forward (F) or reverse (R) genomic orientation followed by transient transfections and luciferase assays. Luciferase activity was normalized to empty vector. Experiments were performed in triplicate and repeated at least three times; errors bars represent the standard error of the mean (s.e.m.). Supplementary Fig. 4 contains luciferase results for one additional cell line per tumour type (MIA PaCa-2, UACC1113, NTERA-2, 2102Ep and NCI-H460).