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Primers used in the study
| Gene | Sequence (5′ to 3′) | Product size |
|---|---|---|
| GAPDH-F | ACTTTGGCATCGTGGAAGGG | 128 bp |
| GAPDH-R | TGCAGGGATGATGTTCTGGG | |
| Kiss1-F | GGTATGCAGAGAGCAAGCCT | 122 bp |
| Kiss1-R | GATCAGGCGACTGCGGG | |
| GnRH-F | CACTGGTCCTATGGGTTGCG | 149 bp |
| GnRH-R | TCCCTAAGAGGTGAACGGGG |
