Table 1.

Optimization of working concentration

Coating antibody		10 μg/mL			5 μg/mL			1 μg/mL			0.1 μg/mL
CAg (ng/mL)		0	1	50	0	1	50	0	1	50	0	1	50
D	1:100	33,506	39,216	614,441	30,142	35,891	640,496	27,993	41,092	635,737	32,337	29,902	40,606
	1:200	27,232	36,745	495,871	29,246	36,645	619,561	25,237	32,604	507,215	28,922	27,300	36,226
	1:300	26,143	31,833	344,716	27,453	34,398	427,663	22,174	33,261	388,885	27,790	27,253	31,046
	1:400	25,002	31,137	240,503	27,130	30,330	247,904	22,373	32,398	192,264	28,985	28,918	29,168

D The dilution ration of QDs-mAb probe