Table 1.
The IC50 values of LAMF, LAMR, morin, and chlorogenic acid in various antioxidant assays (μg/mL or μM)
| Assays | LAMF | LAMR | morin | chlorogenic acid | luteolin | Trolox |
|---|---|---|---|---|---|---|
| PTIO• Scavenging | 6609.7 ± 756.6d | 4286.9 ± 84.9c | 103.4 ± 0.9b (344.6 ± 2.9C) | 99.6 ± 2.3b
(281.4 ± 6.5B) |
ND | 40.6 ± 6.1a (162.2 ± 24.2 A) |
| DPPH•-scavenging | 208.7 ± 3.0d | 97.3 ± 3.1c | 8.2 ± 0.7b
(27.1 ± 2.2 D) |
5.6 ± 0.01a
(15.8 ± 0.03B) |
3.2 ± 0.1a
(11.1 ± 0.2A) |
4.5 ± 0.1a
(18.1 ± 0.4 C) |
| ABTS• scavenging | 73.5 ± 5.8c | 34.4 ± 0.1b | 4.2 ± 0.2a
(13.9 ± 0.6 B) |
5.8 ± 0.3a
(16.4 ± 0.9 B) |
2.6 ± 0.1a
(9.0 ± 0.2A) |
1.9 ± 0.6a
(7.7 ± 2.6A) |
| FRAP | 277.0 ± 3.1c | 191.9 ± 5.2b | 5.0 ± 0.2a
(16.7 ± 0.8 B) |
4.9 ± 1.0a
(13.9 ± 2.7A) |
5.8 ± 0.1a
(20.1 ± 0.2C) |
7.0 ± 0.4a
(28.1 ± 1.7 D) |
| Cu2+-reducing | 212.5 ± 7.0c | 123.2 ± 0.9b | 14.1 ± 0.04a
(46.5 ± 0.1 B) |
12.5 ± 0.6a
(35.2 ± 1.6 A) |
10.2 ± 0.3a
(36.8 ± 0.9A) |
15.7 ± 0.6a
(62.7 ± 2.5 C) |
The IC50 value is defined as the concentration for a 50% effect and is expressed as the mean ± SD (n = 3). The IC50 values without parentheses are in μg/mL units; The IC50 values in parentheses are in μM units. Mean IC50 values in μg/mL units with different superscripts (a, b) in the same row are significantly different (p < 0.05), while those with the same superscripts are not significantly different (p < 0.05). Mean IC50 values in μM units with different superscripts (A, B) in the same row are significantly different (p < 0.05), while those with the same superscripts are not significantly different (p < 0.05). FRAP ferric ion reducing antioxidant power; ABTS, 2,2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid; DPPH, 1,1-diphenyl-2-picryl-hydrazl; PTIO, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide; LAMF, lyophilized aqueous extract of Mori Fructus; lyophilized aqueous extract of Mori Ramulus. Trolox acted as the positive control. The dose-response curves are listed in Additional file 1