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. 2017 May 2;17:319. doi: 10.1186/s12879-017-2418-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Effect of enzymatic digestion of cell GAGs in bacterial adherence to lung cells. a-b Effect of pre-treatment with GAG lyases: heparinase I and III, chondroitinase ABC and all enzymes simultaneously on bacterial adherence to A549 cells (a) and MRC5 cells (b). c Relative differences in mean bacterial adherence between lung cells treated with each lyase individually, and with a combination of them all. Data were normalized using the values for adhesion of bacteria to non-treated cells, and spreads represent standard deviation. Statistically significant differences are denoted by * for p < 0.05, ** for p < 0.01, and *** for p < 0.001