Figure 4.

Specific binding of mutant peptide of TP53 to HLA-A*02:01. Blank control: FITC-goat anti-mouse IgG + T2 cells; negative control: human beta-2-microglobulin were incubated with T2 cells overnight at 37°C + W6/32 + FITC-goat anti-mouse IgG; wide-type (WT) peptide binding affinity analysis: WT peptide (GMNRRPILTII) and human beta-2-microglobulin were incubated with T2 cells overnight at 37°C + W6/32 + FITC-goat anti-mouse IgG; mutated peptide binding affinity analysis: mutated peptide (GMNWRPILTII) and human beta-2-microglobulin were incubated with T2 cells overnight at 37°C + W6/32 + FITC-goat anti-mouse IgG.