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. 2017 May 2;17:307. doi: 10.1186/s12885-017-3298-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — GLA blocks Wnt/β-catenin signaling in MDA-MB-231 cells. MDA-MB-231 cells were exposed to 10 or 20 μM GLA for 48 h. a Western blot analyses relative protein levels of Wnt1, non-phospho (active) β-catenin, and β-catenin. b Expression of Wnt1 and β-catenin (mRNAs) were analyzed by the quantitative real-time polymerase chain reaction (qRT-PCR) (mean ± SD, n = 3). c Immunofluorescence assay analyses the nuclear translocation of β-catenin. d Expression of TCF/LEF4 (mRNAs) analyzed by qRT-PCR (mean ± SD, n = 3). ^** P < 0.01 and ^*** P < 0.001 compared with the control cells

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