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. Author manuscript; available in PMC: 2018 Apr 20.
Published in final edited form as: Mol Cell. 2017 Apr 6;66(2):194–205.e5. doi: 10.1016/j.molcel.2017.03.003

Figure 5. Ribosomes Accumulate at Stop Codons and in 3′ UTRs in eIF5Ad Cells.

Figure 5

(A) Metagene analysis of translation termination. Average ribosome occupancy from all genes aligned at their stop codons for WT and eIF5Ad cells. Arrow denotes stacked ribosomes ~30 nt upstream the stop codon peak.

(B) Overlay and close-up view of (A), showing accumulated ribosomes in 3′ UTRs in eIF5Ad cells.

(C) Similar to (A), metagene plot of stop codons for WT and eIF5Ad lysates treated with high-salt buffer.

(D) Similar to (B), close-up view of 3′ UTR regions for WT and eIF5Ad lysates after high-salt wash. See also Figure S5.