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. Author manuscript; available in PMC: 2018 Apr 20.
Published in final edited form as: Mol Cell. 2017 Apr 6;66(2):194–205.e5. doi: 10.1016/j.molcel.2017.03.003

Figure 6. eIF5A Stimulates eRF1-Mediated Peptidyl Hydrolysis.

Figure 6

(A) Schematic for in vitro termination assays. Met-Phe-Lys elongation complexes containing an A-site stop codon (UAA) are reacted with eRF1:eRF3:GTP in the presence and absence of eIF5A. Time points are quenched with formic acid, peptide products resolved by electrophoretic TLCs, and rates quantified.

(B) Rates of peptidyl hydrolysis by eRF1:eRF3 in presence and absence of eIF5A and hypusination modification. Error bars represent the standard deviation from at least three replicate experiments. See also Figures S6 and S7.