REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mAID	MBL International Corporation	M214-3
Anti-Pgk1 monoclonal	Life Technologies-Novex	459250
Anti-eIF5A	Dr. Thomas Dever	Saini et al., 2009
Chemicals, Peptides, and Recombinant Proteins
Cycloheximide	Sigma-Aldrich	C1988
SuperaseIn	Ambion	AM2694
RNase I	Ambion	AM2294
Superscript III	Invitrogen	56575
GlycoBlue	ThermoFisher	AM9515
Protease Inhibitor Cocktail	Sigma-Aldrich	P8215
IAA (Indole-3-acetic acid sodium salt)	Sigma-Aldrich	I5148
T4 Polynucleotide Kinase	New England Biosciences	M0201L
T4 RNA Ligase 2, Truncated	New England Biosciences	M0242L
CircLigase ssDNA Ligase	Epicenter	CL4115K
Zeba spin desalting column (2 mL)	ThermoFisher	89889
TLC cellulose plate	EMD Millipore	1055770001
Ni-NTA agarose	QIAGEN	30210
HiPrep 26/60 Sephacryl S200	GE Healthcare Life Sciences	17119501
Superose Increase 6 10/300GL	GE Healthcare Life Sciences	29091596
tRNAPhe (yeast)	Sigma	R4018
Initiator tRNAiMet (yeast)	tRNA Probes (College Station, TX)	MI-03
Lys-tRNALys (yeast)	tRNA Probes (College Station, TX)	L-60
Cys-tRNACys (yeast)	tRNA Probes (College Station, TX)	C-60
EasyTag L-35S-Methionine	Perkin-Elmer	NEG709A005MC
Critical Commercial Assays
Ribo-Zero Gold rRNA Removal Kit (Yeast)	Illumina	MRZY1306
IMPACT Kit	New England Biosciences	E6901S
BL21-CodonPlus (DE3)-RIPL	Agilent	NC9122855
Deposited Data
Raw and analyzed data	This paper	GEO: GSE89704
R64-1-1 S288C sacCer3 Genome Assembly	Saccharomyces Genome Database Project	http://downloads.yeastgenome.org/sequence/S288C_reference/genome_releases/
Yeast ncRNA Gene Database	Saccharomyces Genome Database Project	http://downloads.yeastgenome.org/sequence/S288C_reference/rna/archive/rna_coding_R64-1-1_20110203.fasta.gz
Mendeley Database	This paper	http://dx.doi.org/10.17632/nmkd3jbhx7.1
Experimental Models: Organisms/Strains
BY4741	Dharmacon	YSC1048
Δanb1	Dharmacon	Clone ID: 6845
BY4741; AHD1p-OsTIR1-URA3	This paper	yCW30 – WT
Δanb1; Δhyp2; mAID-HYP2-pAG413GAL; AHD1p-OsTIR1-URA3	This paper	yCW33 – eIF5Ad
Oligonucleotides
Please refer to Table S3 for oligonucleotides used in this study		N/A
Software and Algorithms
Bowtie 1.0.0	Langmead et al., 2009	http://bowtie-bio.sourceforge.net
CutAdapt	Martin, 2011	https://cutadapt.readthedocs.io/en/stable/
ImageQuant TL	GE Healthcare Life Sciences	Version 8.1
Kaleidagraph	Synergy Software	Version 4.1.3