Fig. 1.

MiR-30a regulated CRC proliferation and apoptosis both in vitro and in vivo. a CCK-8 assays of SW480 (left) and DLD1 (right) cells with regulated expression of miR-30a. b Detection of apoptosis by TUNEL assays in different miR-30a expression CRC cells. Blue, Hoechst-stained nuclei; green, TUNEL-positive nuclei. Scale bar = 50 μm. c Over-expression of miR-30a in CRC cells blocked G1/S transition. The down-expression of miR-30a cells were activated in G2 phase of the cell cycle. d SW480-NC, SW480-miR-30a, and SW480-miR30a sponge cells were injected into the flanks of nude mice (n = 6). Tumor weights were recorded and assessed. Scale bar = 1 cm. *P < 0.05, **P < 0.01 compared with NC group. The CCK-8 assays were measured in five replicate values for each independent experiment. The TUNEL assays were calculating the numbers of apoptotic cells in one field, and we chose eight fields to calculate for each sample