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. 2017 Apr 28;8:15061. doi: 10.1038/ncomms15061

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Copyright © 2017, The Author(s)

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(a) Domain organization of SOS. Native SOS and various EGFP-fused SOS constructs are shown. (b) Anti-GFP western blotting of SOS constructs from lysates. (c) Intensity profiles of western blot bands. Approximately 90% of SOS^FL and SOS^HDPC were expressed at the correct molecular weight. SOS^Cat and SOS^CatPR were expressed as pure species. (d) Representative single-step photobleaching trace of SOS^FL. (e) Histogram of photobleaching steps for SOS^FL from lysate compared with purified, monomeric EGFP. SOS^FL predominantly exists in a monomeric state. N is ∼200 for each protein.