Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 28;8:15172. doi: 10.1038/ncomms15172

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a,d) Adult females carry the symbionts within two pairs of accessory glands associated with the reproductive system as confirmed by FISH on cross-sections of a female reproductive system (inset). (b,e) Host eggs are covered with a secretion containing Burkholderia bacteria, as revealed by FISH on an egg wash. (c,f) The symbionts colonize invaginations of the cuticle that result in three dorsal compartments represented in red in a three-dimensional (3D) reconstruction of an L. hirta larva. The Burkholderia symbionts were localized by FISH in a cross-section of an L. villosa larva (inset). FISH pictures show Burkholderia-specific staining in red (Burk16S_Cy3), general eubacterial staining in green (EUB338_Cy5), the overlap of these two in yellow and host cell nuclei in blue (DAPI). Scale bars, 20 μm (d) and 50 μm (e,f).