Fig. 2.
chTLR4 mRNA and protein expression in bursa of Fabricius. (a): Dynamic change of mRNA expression of chTLR4 in chicken bursa of Fabricius was determined by qPCR after treatment by gavage for 7 days with or without various dose of APS (APSH:80 mg/kg, APSM:40 mg/kg, APSL:20 mg/kg and normal saline). The chTLR4 mRNA gene expression was normalized to β-actin expression, and standardized to 1.0 in control group. Protein expression of chTLR4 in chicken bursa of Fabricius with the administration of APSL after 5 days was shown in (b). chTLR4 protein expression was observed in the chicken bursa of Fabricius follicular cortex and medulla cells by immunohistochemical staining for three independent samples (b), and (c) treated without rabbit-anti-chicken-chTLR4 (multi-clonal antibody made by our lab) is negative stain. Results represent the means ± SEM of values for five chickens per group.* p < 0.05, ** p < 0.01 and *** p < 0.001 is considered as significant difference