Skip to main content
. 2017 May 4;2(9):e91981. doi: 10.1172/jci.insight.91981

Figure 7. BH4 deficiency impairs noradrenaline synthesis and supplementation of BH4 increases noradrenaline syntheses.

Figure 7

(A and B) Noradrenaline (NA) contents in brown adipose tissue (BAT) (A; n = 6) and urine collected for 48 hours using individual metabolic cages (B; n = 6–8) of hph-1 mice and control mice of the same background at 6 weeks of age. (C) Dihydroxyphenylalanine (DOPA) production in 120 minutes with PC12 cells after incubation with GTPCH I inhibitor (DAHP) for 16 hours (n = 3). (D) DOPA production in PC12 cells in the last 120 minutes in the presence of extracellular tetrahydrobiopterin (BH4) for the total 240 minutes (n = 5). Values are mean ± SEM. Statistical analysis was performed by Student’s t test (A and B). *P < 0.05 vs. control group. Statistical analysis was also performed by 1-way ANOVA with Tukey post-hoc test (C and D). **P < 0.01 vs. values without DAHP or BH4.