Table 1.
Antibody Table
| Peptide/Protein Target | Antigen Sequence (if Known) | Name of Antibody | Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody | Species Raised in; Monoclonal or Polyclonal | Dilution Used |
|---|---|---|---|---|---|
| Myostatin | Mouse GDF8 Asn25-Ser376 | MAb (clone 84214) | R&D Systems, MAB788 | Monoclonal; rat IgG2B | 1:1000 |
| Phospho-Smad2 | Smad3S423/5 | MAb (clone EP823Y) | Abcam, 52903 | Monoclonal; rabbit | 1:2000 |
| Smad2 | Amino terminus hSmad2 | L16D3 | Cell Signaling, 3103 | Monoclonal; mouse | 1:2000 |
| Eukaryotic EF2 | Complete protein | G270 | Nastiuk (Ref. 63) | Rabbit | 1:2000 |
| β-Actin | β-Actin aa 1–14 | AC15 | Sigma, A5441 | Monoclonal; mouse | 1:5000 |
| Rat IgG (H+L) | Rabbit antirat unconjugated | Pierce, 31218 | Rabbit | 1:20 000 | |
| Rabbit IgG (H+L) | Goat antirabbit HRP-conjugated secondary | Pierce, 31460 | Goat | 1:20 000 | |
| Mouse IgG (H+L) | Goat antimouse HRP-conjugated secondary | Pierce, 31430 | Goat | 1:20 000 |