Figure 1. GR is highly expressed in KCs and HSC.

A, C57BL6/J wild-type livers were stained with anti-GR antibody (×200). Arrowheads indicate GR-positive signals in nonparenchymal cells. B, The human liver sections stained with 2 kinds of GR antibody (CAB010435 and HPA004248) provided by The Human Protein Atlas (available at http://www.proteinatlas.org). Arrowhead indicate GR-positive signals in nonparenchymal cells. C, Comparison of GR expression level in isolated HCs, KCs and HSCs. Purity of each cell fraction was confirmed by the expression levels of albumin (Alb), macrophage antigen (CD68), and Col1α1. Asterisks denote statistically significant differences (one-way ANOVA; *, P < .05; ***, P < .001). Normal livers were double-stained with (D) anti-GR antibody (red) and F4/80 antibody (green) or (E) anti-GR antibody (red) and anti-GFAP antibody (green). Nuclei were visualized by DAPI staining (blue). Arrowheads indicate colocalization of GR and cell-specific markers. CV, central vein.