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. 2016 May 4;30(6):660–676. doi: 10.1210/me.2015-1300

Figure 7. Rapamycin treatment and Tg-Nor-1 regulate autophagy activity.

Figure 7.

A, RT-PCR was performed on control and rapamycin treated differentiated C2C12's and the expression of Nor-1 was assayed. B, Western blot analysis was used to assay Beclin-1 expression on WT and Tg-Nor-1 (n = 4) quadriceps femoris protein extracts. C, Relative density normalized to GAPDH of Western blotting band intensity. D, Diagram illustrating function of rapamycin, mTOR, and Nor-1 on autophagy. Statistical calculation was performed using a Student's t test, and P values are indicated on graphs as follows: *, P < .05; **, P < .01; ***, P < .001.