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. 2016 Feb 17;30(4):417–428. doi: 10.1210/me.2015-1217

Figure 1. Effect of palmitate on the expression of FMO isoform mRNA and in human hepatocyte cell lines.

Figure 1.

The mRNA levels of FMO1, FMO3, and FMO5 were measured in (A) HepG2, (B) Huh-7, and (C) IHH cells by qRT-PCR after treatment without (open bars) or with (filled bars) 0.75mM palmitate for 18 hours. Results are shown as mean ± SEM. Student's t test; *, P < .05; **, P < .01 vs untreated cells. D, FMO3 protein was measured by immunoblotting in IHH cells incubated with palmitate and TPA or vehicle as indicated and normalized to 14-3-3 protein levels. Results are mean ± SEM from 3 experiments. Student's t test; *, P < .05; **, P < .01 vs untreated cells; #, P < .05 vs palmitate-treated cells treated with vehicle. E, FMO3 promoter activity was measured by luciferase reporter assay in cells incubated with palmitate and TPA or vehicle as indicated. Results are mean ± SEM from 4 experiments. Student's t test; ##, P < .025 vs palmitate-treated cells treated with vehicle.