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. 2016 Jan 21;30(3):361–371. doi: 10.1210/me.2015-1213

Figure 3. Puerarin improved β-cell function and up-regulating GLP-1R expression.

Figure 3.

A, Real-time PCR analysis from mRNA isolated from the islets cultured in 33.3mM glucose with/without puerarin. Results were normalized to tubulin. Data are shown as mean ± SE from 3 independent experiments. B, GSIS assay was performed in treated mouse islets with/without puerarin. Stimulatory index denotes the amount of stimulated divided by the amount of basal insulin secretion. Data are shown as mean ± SE from 3 independent experiments. C, Representative Western blottings from the islets cultured in 33.3mM glucose with different treatments. D, Western blot analysis of the GLP-1R cellular distribution in Min6 cells after treatment with puerarin. The densitometric analyses of 3 independent experiments are shown; *, P < .05; **, P < .01, 33.3 to 11.1 or 5; #, P < .05, puerarin to DMSO.