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. 2016 Jan 4;30(2):217–233. doi: 10.1210/me.2015-1189

Figure 4. Effects of ER agonists and antagonists on estradiol-mediated induction of Kiss-1 mRNA expression in mHypoA-50 (AVPV) cell line at 24 hours.

Figure 4.

Cells were pretreated for 1 hour in the presence of 1 specific ER antagonist (1μM) or vehicle control followed by treatment with estradiol (100nM), or specific ER agonist (10nM), or vehicle control over a 24-hour time course. Specifically the (A) ERα antagonist MPP, (C) ERβ antagonist PHTPP, (E) ER antagonist ICI-182780, and (F) GPR30 antagonist G-15 were used as pretreatments. The (B) ERα agonist PPT, (D) ERβ agonist DPN, and (G) GPR30 agonist G-1 were used as specific ER agonists. Kiss-1 mRNA expression was determined by quantitative RT-PCR, and levels were normalized to the internal control, histone 3a. Data are expressed as mean ± SEM (n = 4–5 independent experiments); *, P < .05; **, P < .01 Statistical analysis was calculated by two-way ANOVA followed by Bonferroni post hoc test or Student's t test.