Table 1.
Relative mRNA Expression of Selected Genes Within FACS-Separated hrGFP-Positive vs hrGFP-Negative Cells From ChgA-hrGFP Mice
| Cell Type | Gene Family | Gene | n | ΔCT hrGFP+ (CT) | ΔCT hrGFP− (CT) | Enrichment |
|---|---|---|---|---|---|---|
| Stomach | ||||||
| Endocrine | Granins | ChgA | 6 | 74.3 ± 9.19 (20.3) | 0.24 ± 0.08 (28.0) | 762 ± 333 |
| ChgB | 3 | 13.9 ± 1.77 (26.4) | 0.10 ± 0.02 (32.5) | 142 ± 9.78 | ||
| Secretogranin II | 3 | 0.08 ± 0.02 (33.9) | 0.002 ± 0.001 (38.5) | 72.1 ± 42.3 | ||
| Secretogranin III | 3 | 0.41 ± 0.04 (31.5) | 0.02 ± 0.006 (35.5) | 37.3 ± 11.3 | ||
| Secretogranin V | 3 | 1.74 ± 0.23 (29.4) | 0.01 ± 0.002 (35.4) | 139 ± 30.8 | ||
| Vgf | 3 | 1.35 ± 0.21 (29.8) | 0.01 ± 0.006 (36.0) | 190 ± 68.4 | ||
| Prohormone convertases | Prohormone convertase 1/3 | 6 | 0.37 ± 0.03 (27.6) | 0.002 ± 0.0007 (33.8) | 194 ± 68.4 | |
| Prohormone convertase 2 | 6 | 0.52 ± 0.05 (27.1) | 0.02 ± 0.005 (30.7) | 27.3 ± 3.95 | ||
| Peptide hormones | Ghrelin | 3 | 502 ± 58.7 (21.2) | 2.09 ± 0.69 (30.1) | 276 ± 63.1 | |
| Somatostatin | 3 | 184 ± 40.1 (22.7) | 0.53 ± 0.10 (30.1) | 400 ± 167 | ||
| Gastrin | 3 | 6.38 ± 0.42 (27.5) | 6.89 ± 1.38 (26.4) | 0.98 ± 0.14 | ||
| Glucagon | 3 | 0.40 ± 0.1 (31.6) | 0.006 ± 0.002 (36.8) | 82.4 ± 25.1 | ||
| Monoamine production | HDC | 6 | 0.48 ± 0.07 (27.2) | 0.0005 ± 0.0004 (36.1) | 3840 ± 2140 | |
| Tph-1 | 6 | 0.25 ± 0.08 (28.1) | 0.002 ± 0.001 (33.9) | 186 ± 74.9 | ||
| Parietal | H+/K+ ATPase | 6 | 0.33 ± 0.15 (27.7) | 2.56 ± 1.46 (23.7) | 0.18 ± 0.05 | |
| Gastric intrinsic factor | 6 | 3.49 ± 0.64 (24.3) | 3.01 ± 0.61 (23.5) | 1.2 ± 0.14 | ||
| Chief | Pepsinogen F | 6 | 0.24 ± 0.06 (28.2) | 0.45 ± 0.13 (26.2) | 0.55 ± 0.07 | |
| Mucous | Calpain 8 | 6 | 0.003 ± 0.002 (34.6) | 0.009 ± 0.001 (31.9) | 0.92 ± 0.68 | |
| Proximal Small Intestine | ||||||
| Endocrine | Granin | ChgA | 5 | 16.5 ± 4.9 (24.9) | 0.28 ± 0.04 (27.2) | 65.8 ± 22.4 |
| Prohormone convertases | Prohormone convertase 1/3 | 5 | 0.13 ± 0.08 (29.4) | 0.011 ± 0.02 (31.0) | 19.8 ± 10,8 | |
| Prohormone convertase 2 | 5 | 0.017 ± 0.019 (32.3) | 0.003 ± 0.001 (32.9) | 45.4 ± 36.6 | ||
| Peptide hormones | CCK | 5 | 2.25 ± 0.9 (26.7) | 1.36 ± 0.43 (26.7) | 0.79 ± 4.26 | |
| GIP | 5 | 1.49 ± 0.22 (26.7) | 1.79 ± 0.71 (26.4) | 0.83 ± 0.65 | ||
| Monoamine | Tph-1 | 5 | 1.19 ± 0.74 (26.2) | 0.036 ± 0.006 (29.3) | 48.2 ± 26.5 | |
| Enterocytes | Alkaline phosphatase | 5 | 0.74 ± 0.34 (26.8) | 6.69 ± 1.5 (21.7) | 0.26 ± 0.09 | |
| Goblet | Chloride channel calcium-activated 3 | 5 | 0.27 ± 0.05 (28.3) | 1.18 ± 0.25 (24.2) | 0.26 ± 0.05 | |
| Paneth | Lysozyme | 5 | 0.35 ± 0.27 (27.9) | 0.78 ± 0.26 (24.8) | 0.68 ± 0.2 | |
| Colon | ||||||
| Endocrine | Granins | ChgA | 6 | 40.7 ± 9.8 (24.8) | 0.096 ± 0.02 (28.6) | 429 ± 33.7 |
| ChgB | 3 | 30.1 ± 2.9 (27.6) | 0.13 ± 0.03 (30.3) | 260 ± 78.1 | ||
| Secretogranin II | 3 | Not detectable | 0.001 ± 0.00 (37.1) | — | ||
| Secretogranin III | 3 | 0.3 ± 0.14 (35.2) | 0.01 ± 0.003 (35.4) | 44.1 ± 8.2 | ||
| Secretogranin V | 3 | 0.22 ± 0.02 (34.7) | 0.005 ± 0.000 (35.4) | 44.9 ± 4.8 | ||
| Vgf | 3 | 0.57 ± 0.38 (33.9) | 0.007 ± 0.004 (34.9) | 77.2 ± 23.6 | ||
| Prohormone convertases | Prohormone convertase 1/3 | 6 | 0.04 ± 0.008 (33.6) | 0.005 ± 0.001 (31.6) | 10.6 ± 3.3 | |
| Prohormone convertase 2 | 6 | 0.004 ± 0.005 (37.1) | 0.0019 ± 0.016 (34.3) | 3.79 ± 1.79 | ||
| Peptide hormones | GLP-1 | 3 | 0.099 ± 0.03 (36.1) | 0.18 ± 0.05 (29.9) | 0.56 ± 0.29 | |
| PYY | 3 | 0.57 ± 0.38 (33.9) | 0.37 ± 0.14 (28.9) | 1.32 ± 0.4 | ||
| Somatostatin | 3 | 0.20 ± 0.05 (34.9) | 0.03 ± 0.02 (32.3) | 7.64 ± 3.01 | ||
| Monoamine | Tph-1 | 6 | 3.11 ± 1.13 (27.2) | 0.016 ± 0.032 (31.2) | 270 ± 78.5 | |
| Goblet | Chloride channel calcium-activated 3 | 6 | 0.51 ± 0.17 (30.1) | 1.41 ± 0.39 (24.6) | 0.44 ± 0.07 | |
The CT value of each gene has been normalized to the CT value of ywhaz (ΔCT). The mean CT values are listed in parentheses. The enrichment value represents the amount of mRNA in the hrGFP+ cells relative to the amount in the hrGFP− cells. Bold face indicates significant enrichment in hrGFP+ cells; italics indicates significant enrichment in hrGFP- cells assessed by Student's t test.