Figure 1. Ajuba interacts with LXRα.

A and B, co-IP assays of the interaction between Ajuba and LXRα or LXRβ. Myc-Ajuba, Flag-LXRα, or LXRβ was transiently transfected into 293T cells, and 48 hours after transfection, cells were harvested and whole cells extracts were prepared for co-IP assays. Inputs for all co-IP assays were 5% of total lysates, and the experiments were repeated twice. C, Endogenous Ajuba and LXRα interaction in HepG2 cells. Whole cell extracts prepared from 2 × 10⁷ cells were precleared with protein A/G beads for 2 hours, and co-IP assays were performed using Ajuba antibody and normal rabbit IgG. D, LXR agonist T090 stimulates the interaction between Ajuba and LXRα. Myc-Ajuba and Flag-LXRα proteins were cotransfected into 293T cells; 24 hours after transfection, T090 (1μM, 5μM, 10μM) was added into the culture media for another 24 hours, and whole cell extracts were prepared for co-IP assays. All co-IP assays were repeated at least twice.