Figure 2. Identification of the binding domains in Ajuba and LXRα.

A, A schematic diagram shows Ajuba truncation mutants. B, Myc-Ajuba and its truncation mutants, together with Flag-LXRα, were transiently coexpressed in 293T cells, and whole cell extracts were prepared, immunoprecipitated with Flag antibody, and Western blotting were performed with Myc antibody. C, A schematic diagram shows the Flag-tagged deletion mutants of LXRα. The mutants were constructed using the method of the PCR-based site directed mutagenesis, and all mutants were confirmed by DNA sequencing. D, Myc-Ajuba and Flag-LXRα, or its deletion mutants, were coexpressed in 293T cells via transient transfection, and co-IP assays were performed with Myc antibody.