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. 2015 May 28;29(7):1025–1036. doi: 10.1210/me.2015-1027

Figure 1. Tpl2 inhibition in 3T3-L1 adipocytes decreases the induction of COX-2 and the secretion of PGE2 in response to IL-1β, TNF-α, or to a cytokine mix.

Figure 1.

A, COX-2 mRNA expression in 3T3-L1 adipocytes treated without (vehicle, DMSO) or with a pharmacological Tpl2-I (10μM) and then stimulated or not with IL-1β or TNF-α alone (10 ng/mL) or a mix of these 2 cytokines (MIX, 10 ng/mL each) for 4 hours (n = 4 experiments). B, Immunoblot analysis and quantification (n = 4 experiments) of COX-2 protein expression with Hsp90 as loading control in 3T3-L1 adipocytes treated as in A and stimulated for 16 hours with the indicated cytokines. C, PGE2 secretion in the cultured medium after 16 hours of incubation with the cytokines (n = 4 experiments). D, COX-2 mRNA expression in 3T3-L1 adipocytes treated with control siRNA (siC) or Tpl2 siRNA (siT) for 48 hours and then stimulated for 4 hours with the indicated cytokines (n = 4 experiments). E, Immunoblot analysis and quantification (n = 4 experiments) of COX-2 expression with Hsp90 as loading control in siRNA-treated 3T3-L1 adipocytes stimulated for 16 hours with indicated cytokines F, PGE2 secretion in the culture medium after 16 hours of treatment with the indicated cytokines (n = 4 experiments). Data in bar graphs represent mean ± SEM with ‡, P < .05; #, P < .01; ##, P < .001 vs effects in control cells treated with vehicle or siCtrl; *, P < .05; **, P < .01; ***, P < .001 vs IL-1β or MIX effects in vehicle- or siCtrl-treated cells.