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. 2015 May 28;29(7):1025–1036. doi: 10.1210/me.2015-1027

Figure 2. Effect of IL-1β, TNF-α or a cytokine mix on 5-lipooxygenase mRNA expression and on caspase-3 activity in 3T3-L1 adipocytes.

Figure 2.

A, Expression of Alox5 mRNA in 3T3-L1 adipocytes treated without (vehicle, DMSO) or with a pharmacological Tpl2-I (10μM) and then stimulated or not with IL-1β or TNF-α alone (10 ng/mL) or a mix of these 2 cytokines (MIX, 10 ng/mL each) for 4 hours (n = 3 experiments). B, Caspase-3/7 activity in 3T3-L1 adipocytes treated for 4 hours with the indicated cytokines at the same concentration as in A. C, Caspase-3/7 activity in 3T3-L1 adipocytes treated for 16 hours with the indicated cytokines at the same concentration as in A. D, COX-2 mRNA expression in 3T3-L1 adipocytes incubated without or with the pan-caspase inhibitor Z-VAD-FMK (100μM) for 1 hour and then stimulated or not with TNF-α (10 ng/mL) or a mix of TNF-α and IL-1β (MIX, 10 ng/mL each) for 4 hours. Bars are mean ± SEM with *, P < .05; **, P < .01; and ***, P < .001 vs Ctrl condition.