Figure 3. Tpl2 is involved in NF-κB but not CREB phosphorylation in response to IL-1β or the cytokine mix.

3T3-L1 adipocytes were incubated without or with a Tpl2-I (30μM) (A and B) or treated with control or Tpl2 siRNA (siC and siT, respectively) (C). Then, cells were stimulated or not with IL-1β (10 ng/mL) or IL-1β and TNF-α (MIX, 10 ng/mL each) for 20 minutes. Cell were lysed for Western blot analysis with the indicated antibodies. Hsp90 was used as loading control in B and C. Representative immunoblots are shown.