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. 2015 May 28;29(7):1025–1036. doi: 10.1210/me.2015-1027

Figure 4. NF-κB is involved in Tpl2-induced COX-2 mRNA expression in response to IL-1β but not to TNF-α or the cytokine mix.

Figure 4.

A–C, COX-2 mRNA expression in 3T3-L1 adipocytes treated for 48 hours with control siRNA (siCtrl) or p65 NF-κB siRNA (sip65) and stimulated or not for 4 hours with IL-1β (A), the cytokine mix (B), or TNF-α (C) in the absence or presence of a Tpl2-I (10μM). Data are expressed as a percentage of COX-2 mRNA in siCtrl-treated adipocytes stimulated with IL-1β, TNF-α, or the cytokine mix (n = 4 experiments). The inset in A shows a representative immunoblot of the down-regulation of p65 NF-kβ in sip65-treated adipocytes with Hsp90 as loading control. D, PGE2 secretion in the culture medium of 3T3-L1 adipocytes treated with siCtrl or sip65 for 48 hours before stimulation for 16 hours with the indicated cytokines. Data in bar graphs represent mean ± SEM with *, P < .05; **, P < .01; and ***, P < .001.