Skip to main content
. 2015 May 28;29(7):1025–1036. doi: 10.1210/me.2015-1027

Figure 6. Tpl2 activity in adipocytes or in adipose tissue is required for LPS-induced COX-2 expression.

Figure 6.

A, COX-2 mRNA expression (n = 3 experiments) in 3T3-L1 adipocytes incubated without (vehicle, DMSO) or with a Tpl2-I (5μM) and treated for 24 hours with a CM from RAW macrophages activated with LPS (0.5 ng/mL) or with cultured medium containing the same concentration of LPS (control medium, Ctrl). B, COX-2 mRNA expression (n = 4 experiments) in 3T3-L1 adipocytes treated with control siRNA (Adipo siCtrl) or Tpl2 siRNA (Adipo siTpl2) and incubated with CM or control medium (Ctrl) as described in A. C, COX-2 expression with Hsp90 as loading control in the same experimental conditions as in A. Immunoblots from 2 independent experiments are shown. D, Immunoblot analysis and quantification (n = 3 experiments) of COX-2 protein expression with Hsp90 as loading control in the same experimental conditions as in B. E, COX-2 mRNA expression in adipose tissue explants from WT (n = 4) and Tpl2 KO mice (n = 4) incubated with or without LPS (100 ng/mL) for 24 hours. F, COX-2 mRNA expression in epididymal adipose tissue of WT (n = 9) and Tpl2 KO mice (n = 11) ip injected for 5 hours with LPS (2 μg/g of body weight) or NaCl 0.9% as control. Data in bar graphs represent mean ± SEM with *, P < .05 and **, P < .01.