Table 1.
Ligand-Induced Ser-727 Phosphorylation of Hypothalamic STAT-3
| Treatment | Period of Stimulation | mHypoA-2/10 Cells |
mHypoA-2/12 Cells |
||||
|---|---|---|---|---|---|---|---|
| IFN-γ | EGF | BK | IFN-γ | EGF | BK | ||
| Basal | 2.5 min | 1.0 ± 0.2 | 1.0 ± 0.3 | 1.0 ± 0.3 | 1.0 ± 0.1 | 1.5 ± 0.3 | 1.0 ± 0.2 |
| 5.0 min | 1.0 ± 0.2 | 1.1 ± 0.2 | 1.0 ± 0.3 | 1.0 ± 0.2 | 2.1 ± 0.2* | 1.0 ± 0.9 | |
| 10 min | 1.1 ± 0.3 | 3.2 ± 0.4** | 2.5 ± 0.4** | 1.0 ± 0.2 | 3.9 ± 0.3** | 2.1 ± 0.3** | |
| 20 min | 1.1 ± 0.4 | 2.9 ± 0.3** | 2.2 ± 0.2** | 1.1 ± 0.2 | 3.2 ± 0.4** | 2.2 ± 0.2** | |
| 30 min | 1.0 ± 0.6 | 1.2 ± 0.3 | 1.3 ± 0.1 | 1.0 ± 0.4 | 1.1 ± 0.2 | 1.1 ± 0.3 | |
| DMSO | 10 min | ND | 4.3 ± 0.5** | 4.1 ± 0.3** | ND | 4.6 ± 0.4** | 4.3 ± 0.4** |
| BIM-S | 10 min | ND | ND | 4.5 ± 0.3** | ND | ND | 4.1 ± 0.3** |
| PD-184352 | 10 min | ND | 1.1 ± 0.2 | 1.1 ± 0.2 | ND | 1.1 ± 0.4 | 1.2 ± 0.2 |
| AG-1478 | 10 min | ND | ND | 1.1 ± 0.7 | ND | ND | 1.1 ± 0.3 |
Abbreviation: ND, not determined. Western blot data using a phospho-specific antiserum against Ser-727 of the murine STAT-3 protein shown in Figures 3, 4A, and 5B were compiled (n = 5) after quantification by densitometry and normalization to the time point zero (fold of basal) and are expressed as means ± SEM. Asterisks indicate a significant difference to time point zero (1.0).