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. 2014 Dec 4;29(2):187–199. doi: 10.1210/me.2014-1176

Figure 3. Quantification of early pituitary development in dmrt5 morphants.

Figure 3.

A, Analysis of L and W of the anterior shh expression domain in controls (noninjected, wt; scramble MO, Scr) and dmrt5 morphants (Mo) at bud stage did not reveal significant changes (for numbers see text). B, Numbers of six1b (non-pit1-lineage)- and pit-1 (pit-1lineage)-positive cells were counted at 52 hpf. Neither the number of six1b+ nor pit1+ cells differed significantly between dmrt5 morphants and controls. C, Differentiation of somatotropes and thyrotropes was evaluated at 72 hpf based on the expression of GH (gh+) and thyroid-stimulating enzyme (tsh-b+), respectively. No significant changes were observed between morphants and controls. D–F, Determination of apoptotic particles (arrows) in the pituitary lim3 domain (blue) by TUNEL staining (brown). No significant changes were evident in dmrt5 morphants compared with controls. G–I, Analysis of pituitary W, L, and T at 52 hpf after staining with pitx3 revealed no significant changes in dmrt5 morphants. Error bars represent ± SEM, and N is the number of analyzed embryos. Images D and E are ventral views, G and H are dorsal views. Scale bars, 20 μm.