Figure 1. The DamID platform for location analysis of human CBX2.

A, Dam-CBX2 localizes to the nucleus. NT2–D1 cells were transfected with the Dam-V5-CBX2; 48 hours later, cells were detergent extracted and fixed. Dam-V5-CBX2 was visualized by indirect immunofluorescence using a V5 antibody, nuclei were visualized by DAPI. Scale bar, 5 μm. B, Immunoblot analysis of Dam-V5-CBX2 expression in NT2–D1 cells. Whole-cell extracts of cells transfected with same construct as in A were analyzed by immunoblotting for the expression of Dam-V5-CBX2 using a monoclonal antibody against V5; tubulin is used as a loading control. C, Identified enriched regions localize relatively close to TSSs. In a 5-kb window around the TSS are 2 peaks in the distribution. The highest peak reflects binding events in the promoter region. Considering all identified putative binding regions there is a general peak close to a TSS.