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. 2014 Dec 8;29(1):121–129. doi: 10.1210/me.2014-1289

Figure 2. GFP reporter expression in the AVPV of transgenic mice carrying three different lengths of GFP reporter constructs.

Figure 2.

A, Immunofluorescence analysis of kisspeptin and GFP expression in the AVPV. Left and center panels show GFP and kisspeptin immunoreactivities in AVPV sections of representative transgenic mice, respectively, and right panels show computer-aided merged images of immunoreactive signals for kisspeptin (red) and GFP (green) in OVX and E2-implanted OVX (OVX+E2) mice. Insets show the sections at higher magnification. Scale bar, 100 μm. B, Number of GFP-expressing and kisspeptin-immunoreactive cells in the AVPV of transgenic mice. C, The relative GFP signal intensity in the AVPV of transgenic mice. Values are means ± SEM. §, P < .05 vs OVX mice as a main effect.