Figure 5. E2-dependent effects of RNA-processing proteins on mitochondrial RNA levels.
A, RNA was isolated from MCF-7 cells transfected with control NT, TRMT10C, HSD17B10, PRORP, PTCD1, or ELAC2 siRNA in the presence or absence of 100 nM E2 for 18 hours to determine the steady-state levels of mitochondrial transcripts by Northern blotting. Nuclear 18S rRNA abundance was used as an internal control for total RNA loading. Data shown are typical results repeated on three independent RNA preparations. Each blot was probed for the different RNA species by stripping and reprobing. B, Northern blot showing the effects of RNA processing protein knockdown on the steady-state levels of tRNAIle in the presence and absence of 100 nM E2.