Figure 6. E2-dependent effects on mitochondrial RNA processing.
RNA was isolated from MCF-7 cells transfected with control NT, TRMT10C, HSD17B10, PRORP (A) PTCD1, or ELAC2 (B) siRNA in the presence or absence of 100 nM E2 for 18 hours to determine the effects on mitochondrial RNA processing by qRT-PCR relative to control NT siRNA-treated cells and normalized to 18S rRNA. Data shown are typical results repeated on three independent RNA preparations. Data are means ± SD of three separate experiments. P < .05 is marked with an asterisk.