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. 2014 Oct 29;29(1):140–152. doi: 10.1210/me.2014-1301

Figure 2. BMPR-II and ActR-IIB, but not ActR-IIA or TβR-II, enhance ALP activity in C2C12 cells in cooperation with mutant ALK2.

Figure 2.

A–D, BMPR-II and ActR-IIB induced ALP activity in cooperation with mutant ALK2. C2C12 cells were cotransfected with 100 ng of WT ALK2 (closed triangles), ALK2(R206H) (open circles), ALK2(G325A) (closed squares), or a mock vector (x) and with increasing amounts (0–100 ng) of BMPR-II (A), ActR-IIA (B), ActR-IIB (C), or TβR-II (D). The total amount of transfected DNA was adjusted to 200 ng/well using an empty vector. ALP activity was determined on day 3. The data are expressed as the mean ± SD (n = 3).