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. 2014 Oct 29;29(1):140–152. doi: 10.1210/me.2014-1301

Figure 4. The kinase activity of BMPR-II and ActR-IIB is essential for the enhancement of mutant ALK2.

Figure 4.

A–D, Kinase activity-deficient type II receptors failed to enhance mutant ALK2. ALK2(R206H) (A) and ALK2(G325A) (B) were cotransfected in C2C12 cells with WT or KR BMPR-II (A) and ActR-IIB (B), respectively. ALP activity was determined on day 3. The data are expressed as the mean ± SD (n = 3). *, P < .05 and **, P < .01 (ANOVA). Whole-cell lysates prepared from the transfected cells were analyzed via a Western blot analysis using antibodies against phospho-Smad1/5, V5-tag (ALK2), FLAG-tag (type II receptors), Smad1, and tubulin (C and D).