Table 2.

S1P Receptor Subtype Expression in H9c2 Cells^a

	S1P1	S1P2	S1P3	GAPDH
Primary cardiac muscle cells
Ct	17.44	17.97	17.28	13.26
ΔCt	4.18 ± 0.5	4.71 ± 0.6	4.02 ± 0.7
H9c2 cells
Ct	21.89	20.00		14.52
ΔCt	7.37 ± 0.8	5.48 ± 0.7

^aS1P receptor subtype expression was evaluated form total RNA of primary cardiac muscle and H9c2 cells after reverse transcription and real-time PCR amplification using specific primers as reported in Materials and Methods. ΔCt values are reported as mean ± SEM for independent experiments performed in duplicate.