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A, Histone mRNA levels were determined by qRT-PCR in control MEFs and in cells treated with T3 for 48 hours. B, Cells were treated with or without T3 for 24 hours before addition of 50 μg/mL cycloheximide (CHX). H3 levels were analyzed by Western blotting at the times indicated for CHX treatment. α-Tubulin (α-TUB) was used as a loading control.
