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. 2014 Nov 25;29(1):68–75. doi: 10.1210/me.2014-1235

Figure 5. EIF4G2 induction is involved in TH-induced expression of histones.

Figure 5.

A, Relative mRNA levels of Slip1, Eif4g1, and Eif4g2 between T3-treated (24 hours) and untreated MEFs. B, Protein levels of EIF4G2 in treated (24 hours) and untreated cells. C, EIF4G2 levels in control and T3-treated THR-knockout MEFs expressing either THRA or THRB. D, EIF4G2 levels in MEFs and HTC-116 cells treated with T3 or GC-1, as indicated. E, Effect of EIF4G2 depletion by means of siRNA on histone H3 expression. MEFs were transfected with the siRNA and treated with T3 for 48 hours. F, Eif4g2 promoter sequence (−650 to −350) showing the 2 putative TRE hemisites found (in red) and in bold the sequence of the primers used in the ChIP experiments. Cells were treated for 2 hours with T3 and analyzed by ChIP with normal IgGs, histone H3, and THRB antibodies. The amplified DNA corresponds to the −647/−352 promoter region and to a proximal promoter region (−199/−41) used as a negative control.