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. 2014 Nov 12;28(12):1961–1970. doi: 10.1210/me.2014-1135

Figure 7. TA3 promotes cerebellar development and cortical myelination in Mct8/Oatp1c1-dKO mice.

Figure 7.

Mct8/Oatp1c1-dKO (M/O dKO) mice were daily injected with TA3 at a dose of either 50 ng/g BW (50) or 400 ng/g BW (400) between P1 and P12. As controls, WT and M/O-dKO mice were treated with saline. Visualization of PC dendrites on sagittal cerebellar sections by calbindin immunostaining (A, upper row) revealed a reduced PC arborization in M/O-dKO mice, whereas TA3 treatment visibly stimulated dendritogenesis. Coronal forebrain sections of the same animals were immunostained with an antibody against MBP and revealed a significantly reduced immunoreactivity (IR) in the cerebral cortex of saline-treated M/O-dKO mice, whereas animals receiving TA3 exhibited elevated MBP IR. However, quantification of the thickness of the molecular layer (ML) as well as of the cortical MBP IR revealed that only the application of a high dose of TA3 was sufficient in normalizing both parameters in Mct8/Oatp1c1-dKO animals (B). Scale bar in A, 40 μm (upper row) and 80 μm (lower row). *, P < .05; **, P < .01; ***, P < .001. Abbreviation: AU, arbitrary unit.