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. 2014 Jul 29;28(10):1656–1669. doi: 10.1210/me.2014-1117

Figure 5. Genomic location of H3K27ac- or H3K4me3-increased regions and the up-regulation of gene expression by decidualization.

Figure 5.

A, Up-regulated genes by decidualization stimuli were classified into 4 groups: 1) genes without H3K27ac- and H3K4me3-increased regions, 2) genes with H3K27ac- or H3K4me3-increased regions in the proximal promoter region, 3) genes with H3K27ac- or H3K4me3-increased regions in the distal promoter region, and 4) genes with H3K27ac- or H3K4me3-increased regions in both the proximal and distal promoter regions. The distal promoter region includes both distal upstream promoter (more than 3 kb upstream from the TSS) and distal downstream promoter (more than 3 kb downstream from TSS). The numbers of genes in each group are shown. B, The fold changes in mRNA levels of the up-regulated genes by decidualization stimuli were compared among the 4 groups in each individual. The fold changes in mRNA levels were calculated by dividing the dESCs (RPKM+1) by the non-dESCs (RPKM+1) obtained from RNA-seq. Box plots show the distribution of log2-transformed fold change value of each group: genes without H3K27ac- and H3K4me3-increased regions (no), genes with H3K27ac- or H3K4me3-increased regions in the proximal promoter region (proximal+), in the distal promoter region (distal+), and in both proximal and distal promoter regions (proximal+, distal+). Data from each individual (case 1 and case 2) are shown. Solid bars of boxes display the interquartile ranges (25%–75%) with an intersection as the median. The whiskers extending outside the boxes correspond to the lowest and highest data within 1.5-fold interquartile range from the upper and lower quartile. P values are calculated using a Mann-Whitney U test followed by Bonferroni correction. a, P < .01 vs no; b, P < .05 vs proximal+; c, P < .01 vs distal+.