Table 1.

Gene Expression Profile in FG Stage Follicles Isolated From the Ovaries of Wild-Type (lhb^+/+ or lhr^+/+) and Homozygous (lhb^m/m or lhr^m/m) Mutant Female Zebrafish

Gene	lhbm/m	T	lhrm/m	T
Steroidogenesis
mprb	1.84 ± 0.22a	↑	1.39 ± 0.22d
npr	0.37 ± 0.05c	↓	0.12 ± 0.05b	↓
er2a	0.74 ± 0.04a	↓	1.21 ± 0.14d
star1	0.27 ± 0.03a	↓	1.15 ± 0.18d
p450scc	0.42 ± 0.03b	↓	1.13 ± 0.10d
p450c17a1	0.37 ± 0.02c	↓	1.03 ± 0.05d
17b-hsd3	0.41 ± 0.02a	↓	1.28 ± 0.22d
Growth factors
egf	0.27 ± 0.05a	↓	0.72 ± 0.32d
igf3	0.25 ± 0.02a	↓	1.23 ± 0.34d
inhbb	0.50 ± 0.07a	↓	0.85 ± 0.11d
Ovulation
plg	10.17 ± 2.55a	↑	0.83 ± 0.19d
plat	2.70 ± 0.36c	↑	1.85 ± 0.12c	↑
plaua	3.06 ± 0.46a	↑	3.00 ± 1.03a	↑
plaub	0.41 ± 0.06b	↓	2.13 ± 0.20c	↑
ptger4b	0.89 ± 0.09d		2.11 ± 0.17c	↑
ptgs2a	9.74 ± 3.42b	↑	3.16 ± 0.28c	↑
ptgs2b	2.06 ± 0.18a	↑	1.34 ± 0.22d
timp2b	9.75 ± 3.43b	↑	3.22 ± 0.64b	↑
mmp2	1.65 ± 0.33d		1.80 ± 0.29a	↑
mmp14b	1.06 ± 0.10d		3.50 ± 0.59c	↑
mmp15a	0.97 ± 0.10d		1.86 ± 0.34a	↑
mmp15b	0.67 ± 0.04a	↓	1.65 ± 0.39d

Significant regulation of several genes involved in steroidogenesis, growth factors regulating oocyte maturation, and genes related to ovulation was found between the wild-type and mutants. The expression levels were normalized to that of ef1a. The data are presented as the ratio of mean values by comparing mutant to wild type (n = 6 for lhb and n = 8 for lhr). T indicates gene expression trend: ↑, up-regulation of gene expression; ↓, down-regulation of gene expression. The full names of the genes and the primers used in the real-time PCR are listed in Supplementary Table 1.

Statistical significance: ^a P < .05; ^b P < .01; ^c P < .001; ^d no statistical significance compared with the wild type.